EC Number   |
pH Stability |
pH Stability Maximum |
Reference |
|---|
   3.4.22.15 | 3.5 |
6.6 |
over 50% activity remaining, profile overview |
753600 |
| 3.4.22.15 | 3.6 |
6.2 |
activity of cathepsin L increases from pH 3.6 to 6.2 and is independent of the calcium concentration, The rate of trypsin inactivation by cathepsin L is thus approximately 10 times faster at pH 4.0 than at pH 5.5 |
712089 |
| 3.4.22.15 | 4 |
7 |
at pH 4.0 and 5.0 activity decreases slowly and remains at 38.86% and 48.54%, respectively, after incubation at 45°C for 120 min, at pH 7.0, activity of cathepsin L decreases sharply to about 10% residual activity after incubation at 45°C for 10 min |
679950 |
| 3.4.22.15 | 4 |
9 |
after incubation for 2 h at 37°C in AMT buffers (100 mM sodium acetate, 100 mM MES, 200 mM Tris, 1 mM EDTA), the isoform CatL1G shows residual activities of about 30% at pH 4.0, about 75% at pH 5.0, about 85% at pH 6.0, about 45% at pH 7.0, and about 10% at pH 8.0-9.0. Isoform CatL1G retains 25% activity after 2 h at pH 7.5, but retains over 80% activity over 4 h at pH 5.5 and 50% over 24 h |
717293 |
| 3.4.22.15 | 4 |
9 |
isoform CatL5 undergoes significant autoprocessing if the pH of the yeast media is allowed to drop below pH 6.0. After incubation for 2 h at 37°C in AMT buffers (100 mM sodium acetate, 100 mM MES, 200 mM Tris, 1 mM EDTA), the isoform CatL5 shows residual activities of about 30% at pH 4.0-5.0, about 60% at pH 6.0, about 85% at pH 7.0, about 100% at pH 8.0, and about 60% at pH 9.0. Isoform CatL5 and mutant CatL5 L69W retain more than 90% activity after 4 h incubation at pH 7.5 and about 40% activity after incubation for 24 h |
717293 |
| 3.4.22.15 | 4.5 |
5.5 |
37°C, 24 h, more than 70% of the activity is retained |
647919 |
| 3.4.22.15 | 4.5 |
6.5 |
37°C, 1 h, stable |
647929 |
| 3.4.22.15 | 5 |
- |
40 min, 10% loss of activity |
647903 |
| 3.4.22.15 | 5 |
6.5 |
stable |
647927 |
| 3.4.22.15 | 5 |
8.5 |
the enzyme remains stable at neutral and alkaline pH (8.5), its activity is greatly decreased at pH 5.0 (lysosomal pH) and below |
710915 |
