EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
2.2.1.6 | 0 |
- |
freezing and subfreezing temperatures are severely damaging |
395896 |
2.2.1.6 | 30 |
- |
1 hour, no loss of activity |
658566 |
2.2.1.6 | 37 |
- |
half-life: 7.5 min, without substrate and cofactors |
395890 |
2.2.1.6 | 40 |
- |
the activity above 40°C is less than 20% and then completely decreases at higher temperatures |
733744 |
2.2.1.6 | 45 |
- |
15 min, 69% loss of activity, isoenzyme I, slight stimulation of activity of isoenzyme III |
395887 |
2.2.1.6 | 50 |
- |
30 min, stable |
395923 |
2.2.1.6 | 50 |
- |
half-life of recombinant C-terminally His-tagged enzyme is 81 h, comparison with half-lives of mutant enzymes, overview |
733658 |
2.2.1.6 | 50 |
60 |
at 50°C and 60°C, the enzyme shows a half-life of 81 h and 16 h, respectively |
733658 |
2.2.1.6 | 60 |
- |
30 min, stable |
395912 |
2.2.1.6 | 60 |
- |
recombinant wild-type enzyme is highly stable up to 100 min, while mutant enzyme loses over 80% activity within 100 min |
735039 |