EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.7.1.17 | -999 |
- |
the recombinant enzyme is an extremely stable indigo reductase, analysis of the structure of this highly thermostable indigo reductase and identification of the key amino acid residues responsible for the thermostability |
763965 |
1.7.1.17 | 10 |
40 |
purified recombinant enzyme, pH 7.0, 0.1 M sodium phosphate buffer, 30 min, stable at |
743106 |
1.7.1.17 | 20 |
100 |
purified recombinant wild-type enzyme, completely stable, while the mutant shows loss of 50% activity at about 55°C and inactivation at 90°C |
763965 |
1.7.1.17 | 30 |
- |
stable for 1 month |
744137 |
1.7.1.17 | 45 |
- |
stable for at least 1 h |
744136 |
1.7.1.17 | 55 |
- |
purified recombinant enzyme, unfolding temperature |
743106 |
1.7.1.17 | 65 |
- |
1 h, more than 50% residual activity |
744136 |
1.7.1.17 | 65 |
- |
stable for 1 h |
744137 |
1.7.1.17 | 70 |
- |
stable for 30 min |
744571 |
1.7.1.17 | 71 |
- |
apparent melting temperature |
763801 |