EC Number |
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2.1.1.33 | coexpression of both METTL1 and WDR4 in Saccharomyces cerevisiae |
2.1.1.33 | expressed in a wheat germ cell-free translation system, active Trm8-Trm82 heterodimer is only synthesized under conditions, in which both Trm8 and Trm82 mRNAs are cotranslated |
2.1.1.33 | expressed in Escherichia coli strain BL21 (DE3) Rosetta 2 |
2.1.1.33 | expression in Escherichia coli |
2.1.1.33 | ORF YDL201w encodes Trm8 and ORF YDR165w encodes Trm82, both proteins form a complex with m7G-methyltransferase activity, Trm8 contains a binding domain for S-adenosyl-L-methionine: GXGXG, coexpression of Trm8/Trm82 in Escherichia coli BL21 Codon Plus (DE3) as His6-fusion proteins under control of the Ptac-promoter |