EC Number   |
|---|
    1.8.1.4 | - |
| 1.8.1.4 | bfmBC gene encoding DLD. Ligated into vector pCR2.1, and introduced into Escherichia coli DH5alpha. Insert DNA recovered from the recombinant plasmid ligated into vector pET-28a(+), yielding pET-bfmBC and expressed in Escherichia coli BL21 (DE3) |
| 1.8.1.4 | dihydrolipoamide dehydrogenase component of the pyruvate dehydrogenase multienzyme complex, expression in Escherichia coli |
| 1.8.1.4 | DNA and amino acid sequence determination and analysis of both lipdh genes using RT-PCR, expression of the mitochondrial isozyme as GFP-fusion protein giving green fluorescence and of the apicoplast isozyme fused to acyl-carrier-protein resulting in red fluorescence, expression of truncated mitochondrial and apicoplastic isozymes lacking the putative target sequences as His-tagged proteins in Escherichia coli strain BL21-RIL(DE3), the apicoplast isozyme is expressed at very low levels |
| 1.8.1.4 | DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 1.8.1.4 | expressed in Escherichia coli |
| 1.8.1.4 | expressed in Escherichia coli as a recombinant protein |
| 1.8.1.4 | expressed in Escherichia coli BL21 cells |
| 1.8.1.4 | expressed in Escherichia coli BL21(DE3) |
| 1.8.1.4 | expressed in Escherichia coli BL21(DE3) cells |
