EC Number   |
|---|
    1.5.1.34 | - |
| 1.5.1.34 | cloning of full-length cDNA, expression in COS cells |
| 1.5.1.34 | dicDHPR gene (residues 1-231) cloned into pET-28b expression plasmid with hexahistidine tag and thrombin cleavage site, expression in Escherichia coli BL21 (DE3) |
| 1.5.1.34 | DNA sequence determination and analysis, overexpression in Escherichia coli |
| 1.5.1.34 | expressed as His-tag fusion protein in Escherichia coli |
| 1.5.1.34 | expressed in HEK-293T cells |
| 1.5.1.34 | expression of cloned cDNA in Escherichia coli |
| 1.5.1.34 | expression of wild-type and several mutants in Escherichia coli |
| 1.5.1.34 | expression of wild-type, G19D, W104G and T119insert mutant enzymes in Escherichia coli |
| 1.5.1.34 | expression of wild-type, Y150H, Y150S, Y150F, Y150E, Y150K, G151S and G23D in Escherichia coli |
