EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.4.1.221 | GDP-6-alkynyl fucose + protein |
6-alkynyl fucose is efficiently incorporated onto EGF repeats, TSRs, and N-glycan on Lfng and N-glycans on a number of proteins in crude lysates of CHO cells, e.g. the O-fucosylation site in EGF3 of mouse Notch1 and elongated by Lfng, mass spectrometry analysis, overview. Using the Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC), or click reaction, azido-biotin allows tagging and detection of 6AF-modified proteins |
Mus musculus |
? |
- |
? |
2.4.1.221 | GDP-beta-L-fucose + factor VII EGF |
- |
Cricetulus griseus |
? |
- |
? |
2.4.1.221 | GDP-beta-L-fucose + factor VII EGF |
- |
Homo sapiens |
? |
- |
? |
2.4.1.221 | GDP-beta-L-fucose + protein |
protein O-fucosyltransferases 1 and 2 (PoFUT1 and PoFUT2) are the enzymes responsible for this protein O-fucosylation and selectively glycosylate specific residues in epidermal growth factor-like (EGF) repeats and thrombospondin type I repeats (TSRs). PoFUT1 glycosylates epidermal growth factor-like (EGF) repeats within the consensus sequence C2-X-X-X-X-S/T-C3 |
Homo sapiens |
GDP + ? |
- |
? |
2.4.1.221 | GDP-beta-L-fucose + protein |
protein O-fucosyltransferases 1 and 2 (PoFUT1 and PoFUT2) are the enzymes responsible for this protein O-fucosylation and selectively glycosylate specific residues in epidermal growth factor-like (EGF) repeats and thrombospondin type I repeats (TSRs). PoFUT1 glycosylates epidermal growth factor-like (EGF) repeats within the consensus sequence C2-X-X-X-X-S/T-C3 |
Mus musculus |
GDP + ? |
- |
? |
2.4.1.221 | GDP-beta-L-fucose + protein |
protein O-fucosyltransferases 1 and 2 (PoFUT1 and PoFUT2) are the enzymes responsible for this protein O-fucosylation and selectively glycosylate specific residues in epidermal growth factor-like (EGF) repeats and thrombospondin type I repeats (TSRs). PoFUT1 glycosylates epidermal growth factor-like (EGF) repeats within the consensus sequence C2-X-X-X-X-S/T-C3 |
Caenorhabditis elegans |
GDP + ? |
- |
? |
2.4.1.221 | GDP-beta-L-fucose + protein |
protein O-fucosyltransferases 1 and 2 (PoFUT1 and PoFUT2) are the enzymes responsible for this protein O-fucosylation and selectively glycosylate specific residues in epidermal growth factor-like (EGF) repeats and thrombospondin type I repeats (TSRs). PoFUT2 glycosylates thrombospondin type I repeats (TSRs) containing Ser/Thr residues located in the consensus sequences C1-X-X-S/T-C2 or C2-X-X-S/T-C3 of TSRs of groups 1 and 2 |
Homo sapiens |
GDP + ? |
- |
? |
2.4.1.221 | GDP-beta-L-fucose + protein |
protein O-fucosyltransferases 1 and 2 (PoFUT1 and PoFUT2) are the enzymes responsible for this protein O-fucosylation and selectively glycosylate specific residues in epidermal growth factor-like (EGF) repeats and thrombospondin type I repeats (TSRs). PoFUT2 glycosylates thrombospondin type I repeats (TSRs) containing Ser/Thr residues located in the consensus sequences C1-X-X-S/T-C2 or C2-X-X-S/T-C3 of TSRs of groups 1 and 2 |
Caenorhabditis elegans |
GDP + ? |
- |
? |
2.4.1.221 | GDP-beta-L-fucose + protein |
SN1-like mechanism. PoFUT1 glycosylates epidermal growth factor-like (EGF) repeats within the consensus sequence C2-X-X-X-X-S/T-C3 |
Homo sapiens |
GDP + ? |
- |
? |
2.4.1.221 | GDP-beta-L-fucose + protein |
SN1-like mechanism. PoFUT1 glycosylates epidermal growth factor-like (EGF) repeats within the consensus sequence C2-X-X-X-X-S/T-C3 |
Mus musculus |
GDP + ? |
- |
? |