EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.3.2.B1 | ditrans,ocatacis-undecaprenyl diphosphoryl (GlcNAc-beta-(1->4))MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-Ala-tRNa |
- |
Streptococcus pneumoniae |
ditrans,ocatacis-undecaprenyl diphosphoryl (GlcNAc-beta-(1->4))MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA |
- |
? |
2.3.2.B1 | ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA |
i.e. lipid I |
Streptococcus pneumoniae |
? + tRNA |
- |
? |
2.3.2.B1 | ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA |
i.e. lipid I |
Streptococcus pneumoniae 159 |
? + tRNA |
- |
? |
2.3.2.B1 | ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA |
i.e. lipid I |
Streptococcus pneumoniae Pn16 |
? + tRNA |
- |
? |
2.3.2.B1 | ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA |
- |
Streptococcus pneumoniae |
? + tRNA |
- |
? |
2.3.2.B1 | ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA |
- |
Streptococcus pneumoniae 159 |
? + tRNA |
- |
? |
2.3.2.B1 | ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA |
- |
Streptococcus pneumoniae Pn16 |
? + tRNA |
- |
? |
2.3.2.B1 | more |
a 30-amino acid long sequence within the MurM protein is the main determinant of the specificity of the reaction, the addition of alanine versus serine |
Streptococcus pneumoniae |
? |
- |
? |
2.3.2.B1 | more |
differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla |
Streptococcus pneumoniae |
? |
- |
? |
2.3.2.B1 | more |
MurM acts during the lipid-linked stages of peptidoglycan synthesis.The D-alanyl-D-alanine of the stem peptide and the lipid II N-acetylglucosaminyl group are not essential for substrate recognition. epsilon-Carboxylation of the lysine of the stem peptide is not tolerated. Differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla |
Streptococcus pneumoniae |
? |
- |
? |