EC Number |
Storage Stability |
---|
2.2.1.6 | -20°C or -80°C, not stable |
2.2.1.6 | -20°C, 20% glycerol, stable for 2 months |
2.2.1.6 | -20°C, 50 mM phosphate, pH 8, 50% v/v glycerol, 3 weeks, less than 25% loss of activity |
2.2.1.6 | -20°C, stable for over 1 month |
2.2.1.6 | -25°C, no significant loss of activity after 6 months |
2.2.1.6 | -70°C, 21 days, 15% loss of activity of mutant enzyme A122V, less than 1% loss of activity of mutant enzymes W574S and S653N, 3% loss of activity of mutant enzyme W574L |
2.2.1.6 | -70°C, 3 weeks, loss of 5% of activity |
2.2.1.6 | -70°C, 4% loss of activity after 3 weeks with three freeze-thaw cycles, 8% loss of activity after 5 weeks with five freeze-thaw cycles, 9-10% loss of activity after 6 months with no intervening freeze-thaw cycle |
2.2.1.6 | -80°C, concentrated enzyme solution is indefinitely stable |
2.2.1.6 | 0-4°C, 50 mM potassium phosphate, pH 7.0, 10 mM mercaptoethanol, 1 mM MgCl2, 0.01 mM thiamine diphosphate, 1 month, 20% loss of activity |