1.13.12.7 | renaturation is initiated by diluting the denaturant in 50 mM HEPES-KOH, pH 7.5, 10 mM Mg(OAc)2, 70 mM KOAc, 50 mM imidazole, and 1 mM dithiothreitol without urea, refolding of firefly luciferase from a denatured state is a slow process, its rate and productivity depend on molecular chaperones of the Hsp70 family, Hsp70-dependent refolding restores 55% of the initial enzymatic activity, immobilization leads to a higher refolding yield owing to the prevention of intermolecular aggregation |
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