Refine search

Search Renatured (Commentary)

Search term:

Results 1 - 1 of 1

EC Number	Renatured (Commentary)	Reference
1.1.1.27	denaturation in 6 M guanudine HCl for 2 min, then dilution 100fold in 0.1 M phosphate buffer, pH 7.5, containing 10 mM beta-mercaptoethanol. After standing for 1 h 18% of the wild-type activity is regained, 20% is regained after 12 h. The mutant enzyme with a deletion of 5 N-terminal amino acids is restored to 15% and 17% after 1 h and 12 h renaturation. The mutant with a deletion of 10 N-terminal amino acids can only be restored to 3% of the original activity after 1 h renaturation and at most 7% after 12 h	654791

Results 1 - 1 of 1

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)