EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   3.4.11.1 | 0.0016 |
- |
L-Pro-4-nitroanilide |
pH 7.5, 80°C |
747672 |
| 3.4.11.1 | 0.0021 |
- |
L-Lys-4-nitroanilide |
pH 7.5, 35°C |
747672 |
| 3.4.11.1 | 0.0023 |
- |
L-phenylalanine-4-methylcoumarin-7-amide |
pH 8.0, 37°C, recombinant GST-tagged enzyme |
709864 |
| 3.4.11.1 | 0.0025 |
- |
L-Leu-4-nitroanilide |
pH 7.5, 35°C |
747672 |
| 3.4.11.1 | 0.0027 |
- |
L-leucine-4-methylcoumaryl-7-amide |
pH 8.0, 37°C, recombinant enzyme in presence of Zn2+ and Mg2+, best ion combination at metal binding sites 1 and 2 |
707498 |
| 3.4.11.1 | 0.0032 |
- |
L-Ala-4-nitroanilide |
pH 7.5, 80°C |
747672 |
| 3.4.11.1 | 0.0054 |
- |
L-lysine-4-methylcoumaryl-7-amide |
pH 8.5, 37°C |
718187 |
| 3.4.11.1 | 0.011 |
- |
L-proline-4-methylcoumaryl-7-amide |
pH 8.0, 37°C, recombinant GST-tagged enzyme |
710202 |
| 3.4.11.1 | 0.013 |
- |
L-proline-4-methylcoumarin-7-amide |
pH 8.0, 37°C, recombinant GST-tagged enzyme |
709864 |
| 3.4.11.1 | 0.0218 |
- |
L-Lys-4-nitroanilide |
pH 7.5, 80°C |
747672 |
