EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    2.3.1.31 | 0.0074 |
- |
L-homoserine |
R249M, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C |
687796 |
| 2.3.1.31 | 0.009 |
- |
L-homoserine |
recombinant His-tagged mutant G52A enzyme, pH 8.0, 30°C |
736358 |
| 2.3.1.31 | 0.015 |
- |
L-homoserine |
recombinant His-tagged wild-type enzyme, pH 8.0, 30°C |
736358 |
| 2.3.1.31 | 0.0176 |
- |
L-homoserine |
E237A, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C |
687796 |
| 2.3.1.31 | 0.0228 |
- |
acetyl-CoA |
K47M, 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C |
687796 |
| 2.3.1.31 | 0.0507 |
- |
L-homoserine |
K47M, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C |
687796 |
| 2.3.1.31 | 0.118 |
- |
L-homoserine |
K163M, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C |
687796 |
| 2.3.1.31 | 0.139 |
- |
acetyl-CoA |
E237A, 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C |
687796 |
| 2.3.1.31 | 0.147 |
- |
L-homoserine |
E250A, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C |
687796 |
| 2.3.1.31 | 0.18 |
- |
L-homoserine |
E237Q, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C |
687796 |
