EC Number   |
General Information |
Reference |
|---|
   1.1.1.307 | evolution |
Meyerozyma caribbica and Calamus tenuis xylose reductase have close evolution relationship in Rossmann fold |
762689 |
| 1.1.1.307 | malfunction |
overexpression of endogenous XR enhances xylitol productivity at 40°C by thermotolerant Kluyveromyces marxianus, high-temperature xylose consumption and xylitol production rates of the mKmXYL1 gene-overexpressing strain are compared to those of the parental strain KCTC17555DELTAURA3 |
-, 760372 |
| 1.1.1.307 | metabolism |
D-glucose-induced algal cells exhibit a remarkably increased D-xylose uptake rate. The uptake of D-xylose activates the related metabolic pathway, and the activities of a NAD(P)H-linked xylose reductase XR and NADP+-linked xylitol dehydrogenase XDH are detected in C. sorokiniana. Compared with the culture in the dark, the consumption of D-xylose increases 2fold under light but decreases to the same level with addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea |
-, 740206 |
| 1.1.1.307 | metabolism |
derivatives of D-xylose and D-glucose, in which the hydroxy groups at C-5, and C-5 and C-6 are replaced by fluorine, hydrogen and azide are reduced with up to 3000fold increased catalytic efficiencies. Azide introduced at C-5 or C-6 destabilizes the transition state of reduction of the corresponding hydrogen-substituted aldoses by approx. 4 kJ/mol |
764095 |
| 1.1.1.307 | metabolism |
in anaerobic culture, NAD+ generated in the NAD(P)H-dependent xylose reductase reaction is likely needed in the NAD+-dependent xylitol dehydrogenase reaction, whereas in aerobic culture, the NAD+ generated by oxidation of NADH in the mitochondria is required in the xylitol dehydrogenase reaction, analysis of the relationship between NAD(P)+/NAD(P)H redox balances and metabolisms of xylose or xylitol as carbon sources in aerobic and anaerobic batch cultures of recombinant Saccharomyces cerevisiae in a complex medium containing 20 g/l xylose or 20 g/l xylitol at pH 5.0 and 30°C. Addition of acetaldehyde (an effective oxidizer of NADH) increases the xylitol consumption by the anaerobically cultured strain. Gal2 and Fps1 transport xylitol both inward and outward and play a role in xylitol consumption importing xylitol into the cytosol and exporting it from the cells |
-, 742941 |
| 1.1.1.307 | metabolism |
key enzymes for xylitol production in yeasts are xylose reductase and xylitol dehydrogenase, EC 1.1.1.9, overview |
699368 |
| 1.1.1.307 | metabolism |
one xylose-assimilating pathway consists of xylose reductase (XR, XYL1) and xylitol dehydrogenase (XDH, XYL2, EC 1.1.1.9) from Scheffersomyces stipitis. XR reduces xylose to xylitol by using NAD(P)H as cofactor and XDH further oxidizes xylitol to xylulose using NAD+. While the XR-XDH pathway can offer higher metabolic fluxes than the xylose isomerase (XI) pathway, it accumulates xylitol which is produced due to cofactor imbalance caused by different cofactor requirement between XR and XDH |
-, 760714 |
| 1.1.1.307 | metabolism |
ordered mechanism in which coenzyme binds first and substrate second |
764945 |
| 1.1.1.307 | metabolism |
xylose reductase is the first enzyme in D-xylose metabolism, catalyzing the reduction of D-xylose to xylitol |
-, 721848 |
| 1.1.1.307 | more |
structure homology modeling based on a XR structure from Candida sp. (CaXR) as template (PDB ID 1SM9), analysis of the architecture of the cofactor binding site. Notable CaXR-to-CtXR replacements include N276T, L277R, R280I and Q283S |
-, 761297 |
