EC Number |
---|
3.5.1.2 | - |
3.5.1.2 | acetone precipitation and carboxymethyl-methacrylate column chromatography |
3.5.1.2 | by centrifugation, lysis, nickel affinity chromatography |
3.5.1.2 | by two-step ammonium sulfate precipitation, hydrophobic interaction chromatography on phenyl-Sepharose, anion exchange chromatography on DEAE-cellulose, hydrophobic interaction chromatography on phenyl-Superose and anion exchange chromatography using Mono Q, 762fold |
3.5.1.2 | DEAE Sepharose column chromatography, hydroxyapatite column chromatography, and Sephacryl S200 gel filtration |
3.5.1.2 | DEAE-cellulose column chromatography |
3.5.1.2 | DEAE-Toyopearl column |
3.5.1.2 | glutaminase B |
3.5.1.2 | ion exchange column chromatography and gel filtration |
3.5.1.2 | native enzyme 250fold from strain NYW-81 to homogeneity by two steps of anion exchange chromatography, hydrophobic interaction and hydroxylapatite chromatography, and gel filtration |