EC Number   |
|---|
    2.3.1.31 | - |
| 2.3.1.31 | cells are centrifuged in 50 mM Tris, pH 8.0, sonicated, centrifuged, supernatant loaded to a Ni2+-nitrilotriacetic acid-agarose column, washed, and eluted, eland incubated with protease and dialyzed, reloaded onto a Ni2+-nitrilotriacetic acid-agarose column, dialyzed, loaded onto Superdex-200 size-exclusion fast protein liquid chromatography column with 25 mM HEPES-buffer, pH 7.5 |
| 2.3.1.31 | partial |
| 2.3.1.31 | partial, mutant strain 6 |
| 2.3.1.31 | recombinant from E. coli |
| 2.3.1.31 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion excange chromatography, followed by gel electrophoresis, dialysis, and ultrafiltration |
| 2.3.1.31 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-T1 by nickel affinity chromatography and two different steps of gel filtration |
| 2.3.1.31 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, dialysis and ultrafiltration |
| 2.3.1.31 | using Ni-NTA chromatography |
| 2.3.1.31 | using Ni-NTA-chromatography |
