EC Number |
---|
2.3.1.269 | Ni-NTA column chromatography and Superdex 200 gel filtration |
2.3.1.269 | recombinant His-tagged wild-type enzyme from Escherichia coli strain C41(DE3) and His-tagged Se-Met-labeled Lnt from Escherichia coli strain B834 (DE3), both by nickel affinity chromatography, desalting gel filtration, ion exchange chromatography, and ultrafiltration and gel filtration |
2.3.1.269 | recombinant His8-tagged enzyme from Escherichia coli strain C41(DE3) membrane fraction by nickel affinity chromatography, ultrafiltration, gel filtration, and again ultrafiltration |
2.3.1.269 | recombinant Strep-tagged wild-type and mutant enzymes from Escherichia coli by affinity chromatography and gel filtration |
2.3.1.269 | Strep-Tactin column chromatography |