EC Number   |
|---|
   2.1.1.228 | - |
| 2.1.1.228 | recombinant C-terminally His-tagged enzyme, lacking the N-terminal peptide V2LWILWRP9, from Escherichia coli by metal ion affinity chromatography and anion exchange chromatography |
| 2.1.1.228 | recombinant enzyme |
| 2.1.1.228 | recombinant expression of His-tagged enzyme by affinity chromatography and desalting gel filtration |
| 2.1.1.228 | recombinant expression of His-tagged wild-type and mutant enzymes to near homogeneity by 20 min heat treatment at 70°C and 65°C, respectively, followed by affinity chromatography and desalting gel filtration |
| 2.1.1.228 | recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography |
| 2.1.1.228 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography |
| 2.1.1.228 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through 3C protease, another step of nickel affinity chromatography, followed by gel filtration |
| 2.1.1.228 | recombinant His-tagged full-length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration |
| 2.1.1.228 | recombinant His-tagged isolated N-terminal domain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration |
