EC Number |
---|
2.1.1.165 | - |
2.1.1.165 | gel filtration, anion exchange chromatography, and affinity chromatography on adenosine-agarose |
2.1.1.165 | native enzyme 2700fold to homogeneity by ammonium sulfate fractionation, gel filtration, adenosine affinity chromatography, and a second step of gel filtration |
2.1.1.165 | partial |
2.1.1.165 | the difficulty of solubilization of this membrane-bound labile enzyme is the greatest obstacle to its purification |
2.1.1.165 | the purification is achieved by an 80 to 100% ammonium sulfate precipitation step followed by a high-performance liquid chromatography gel filtration step on a 60 cm by 2.15 cm preparative Bio-Sil SEC-250 column. The column is eluted with 10 mM phosphate buffer, pH 7.0 |