EC Number   |
|---|
   1.8.3.2 | - |
| 1.8.3.2 | from egg white form a riboflavin-binding, protein-deficient strain, several steps |
| 1.8.3.2 | from egg white, preparation of apoprotein |
| 1.8.3.2 | gel filtration |
| 1.8.3.2 | glutathione-Sepharose 4B column chromatography |
| 1.8.3.2 | glutathione-Sepharose column |
| 1.8.3.2 | intact enzyme and proteolytically cleaved enzyme in 30 kDa and 60 kDa fragments |
| 1.8.3.2 | pB119L is located in the insoluble fraction, it can be solubilized by addition of arginine, and a truncated version lacking 16 residues at the carboxy terminus is a soluble protein |
| 1.8.3.2 | recobinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration |
| 1.8.3.2 | recombinant enzyme from Escherichia coli by heat treatment, ethanol precipitation, ion exchange chromatography, and gel filtration |
