EC Number |
---|
1.4.1.13 | - |
1.4.1.13 | of the alpha subunit, using column chromatography on Q-Sepharose and Ultrogel AcA34 |
1.4.1.13 | of the G298A-beta subunit, using column chromatography on Reactive Red or Amicon Red resins and gel filtration on Ultrogel AcA 34 |
1.4.1.13 | of the recombinant enzyme beta subunit, using ammonium sulfate fractionation, affinity chromatography on Reactive Red, ultrafiltration and column chromatography on Ultrogel AcA 54 |
1.4.1.13 | of the recombinant enzyme from overproducing Escherichia coli cells, using ion exchange chromatography on Q-Sepharose, gel filtration on Sephacryl S-300 and affinity chromatography on 2',5' ADP-Sepharose 4B colum |
1.4.1.13 | partial purification using column chromatography on DEAE-cellulose |
1.4.1.13 | partial, using ultrasonic oscillation, ammonium sulfate precipitation, column chromatography on Sephacryl S300 and DE-52 cellulose |
1.4.1.13 | recombuinant enzyme and isolated beta subunit |
1.4.1.13 | using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and Blue-Sepharose |
1.4.1.13 | using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and Sephadex G-200 |