EC Number   |
Application |
Reference |
|---|
    2.3.1.9 | analysis |
assay based on the specific absorbance at 303 nm of the Mg2+-enolate complex of acetoacetyl-Coa |
704027 |
| 2.3.1.9 | biofuel production |
the enzyme catalyses the condensation of two acetyl-coenzyme A molecules to form acetoacetyl-CoA in a dedicated pathway towards the biosynthesis of n-butanol, an important solvent and biofuel |
736876 |
| 2.3.1.9 | synthesis |
engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. An Escherichia coli strain with an activated sleeping beauty mutase (Sbm) operon is used to generate propionyl-CoA as a precursor. Two acetyl-CoA moieties or acetyl-CoA and propionyl-CoA are condensed to form acetoacetyl-CoA and 3-ketovaleryl-CoA, respectively, by functional expression of beta-ketothiolases from Cupriavidus necator (i.e. PhaA and BktB). The resulting thioester intermediates are channeled into the polyhydroxyalkanoate biosynthetic pathway through functional expression of acetoacetyl-CoA reductase (PhaB) for thioester reduction and PHA synthase (PhaC) for subsequent polymerization. High-level poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production ranging from 3 to 19 mol%, can be achieved |
-, 758384 |
| 2.3.1.9 | synthesis |
the enzyme has biotechnological potential in haloarchaea for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with controllable 3-oxopentano content, from unrelated cheap carbon sources |
-, 726744 |
| 2.3.1.9 | synthesis |
upon overexpression of acetyl-CoA acetyltransferase (genes AtoAD) in an engineered Escherichia coli strain, carrying polyhydroxyalkanoate synthetic genes bktB, phaB, and phaC and with propionate as a cosubstrate, the 3-hydroxyvalerate faction in Polyhydroxyalkanoate increases up to 7.3fold compared to a strain without genes AtoAD expressed in trans (67.9 mol%). Overexpression of AtoAD decreases the amount of acetyl-CoA and increases the propionyl-CoA/acetyl-CoA ratio, ultimately resulting in an increased 3-hydroxyvalerate fraction in polyhydroxyalkanoate. Synthesis of poly(3-hydroxybutanoate-co-3-hydroxyvalerate) containing 57.9 mol% of 3-hydroxyvalerate is achieved by fed-batch fermentation with propionate |
756208 |
