EC Number   |
Recommended Name |
Application |
|---|
   6.5.1.1 | DNA ligase (ATP) |
analysis |
piezoelectric method for DNA point mutation detection based on DNA ligase reaction and nano-Au-amplified DNA probe |
| 6.5.1.2 | DNA ligase (NAD+) |
analysis |
DNA ligase is an essential reagent in studies on nucleic acid structure and metabolism |
| 6.5.1.2 | DNA ligase (NAD+) |
analysis |
DNA ligase, in combination with polynucleotide kinase, can be used to identify 3'- and 5'-end groups at single-strand interruptions by nearest neighbor analysis |
| 6.5.1.2 | DNA ligase (NAD+) |
analysis |
DNA ligase can be used to determine the ability of other enzymes to act at nicks and gaps in duplex DNA molecules |
| 6.5.1.2 | DNA ligase (NAD+) |
analysis |
DNA ligase can be used to study the primary and secondary structure of DNA molecules |
| 6.5.1.2 | DNA ligase (NAD+) |
analysis |
high-throughput assay for the adenylation reaction of bacterial DNA ligase |
 7.1.1.2 | NADH:ubiquinone reductase (H+-translocating) |
analysis |
kinetic analysis of enzyme using a seff-assembled proteoliposome system containing complex I, Q10, and quinol oxidase (the alternative oxidase, AOX) to recycle Q10H2 to Q10. Complex I is completely rate determining and the Q10 pool is kept oxidized under steady-state catalysis |
| 7.1.1.7 | quinol oxidase (electrogenic, proton-motive force generating) |
analysis |
development of a mCherry-based fluorescent reporter for detection of cytochrome bd expression in Mycobacterium marinum. The expression reporter is suitable for monitoring mycobacterial cytochrome bd expression during mouse macrophage infection and in a zebrafish embryo infection model |
| 7.1.1.9 | cytochrome-c oxidase |
analysis |
cytochrome c oxidase modified electrodes can be used to distinguish amino acid sequence variations in proteins such as cytochrome c. This has potential relevance as a diagnostic for disease states, characterization of electron transfer reactions of cytochrome c isolated from ischemic and control hearts |
| 7.2.2.8 | P-type Cu+ transporter |
analysis |
method to probe Cu flow through human Atox1 and copper chaperone ATP7B proteins when expressed in yeast. |
| 7.5.2.1 | ABC-type maltose transporter |
analysis |
the maltose/maltodextrin transport system of Escherichia coli/Salmonella serves as a model for canonical ATP-binding cassette importers in general |
| 7.6.2.1 | P-type phospholipid transporter |
analysis |
assay for phospholipid flippase activities of plasma membrane-localized P4-ATPases using human cell lines stably expressing isoforms ATP8B1, ATP8B2, ATP11A, and ATP11C |
| 7.6.2.1 | P-type phospholipid transporter |
analysis |
phosphatidylserine-coumarin O-[[(2R)-2-[(9-[5-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]-1H-1,2,3-triazol-4-yl]nonanoyl)oxy]-3-(hexadecanoyloxy)propoxy](hydroxy)phosphoryl]-L-serine exhibits bright fluorescence and good photostability. It should be useful as a blue-emitting fluorescent translocation substrate for extended imaging studies of flippase action in living cells using laser confocal microscopy |
| 7.6.2.2 | ABC-type xenobiotic transporter |
analysis |
evaluation of high-speed ATPase activity assay method by comparing with in vitro transport assay systems using MDR1-transfected MDR1-MDCK cells. The kinetic parameters obtained in ATPase activity assay are not necessarily corresponding with those in in vitro transport assay. The combination of the ATPase activity assay and in vitro transport technologies gives insight into mechanisms |
| 7.6.2.2 | ABC-type xenobiotic transporter |
analysis |
use of in vitro transporter assays for analysis of the role of P-glycoprotein mediated efflux activity. The transwell assay is a valuable tool to evaluate human P-glycoprotein interaction with compounds for assessing brain penetration of new chemical entities |
| 7.6.2.2 | ABC-type xenobiotic transporter |
analysis |
use of in vitro transporter assays for analysis of the role of P-glycoprotein mediated efflux activity. The transwell assay is a valuable tool to evaluate P-glycoprotein interaction with compounds for assessing brain penetration of new chemical entities |
| 7.6.2.2 | ABC-type xenobiotic transporter |
analysis |
use of pharmacophore models for the prediction of binding to P-glycoprotein |
