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EC Number Organic Solvent Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.14.20.1urea purification is achieved by an anion-exchange-chromatographic step in the presence of denaturing concentrations of urea. The main obstacle to converting the homogenous unfolded protein into the active enzyme is a urea-dependent aggregation during refolding that led to irreversible enzyme inactivation 440375
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