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EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Reference
1.2.1.31
-999
-
wild-type ALDH7A1 in the absence of any substrates is subjected to SEC-MALS-SAXS analysis using three nominal loading concentrations in the range of 2-8 mg/ml (18-72 microM by dimer Mr). Results of the MALS experiment show the average in-solution Mr increases from 134 kDa to 158 kDa with increasing protein load concentration, consistent with a concentration-dependent dimer-tetramer equilibrium. For reference, the dimer Mr is 111 kDa. Wild-type ALDH7A1 in the absence of any substrates is subjected to SEC-MALS-SAXS analysis using three nominal loading concentrations in the range of 2-8 mg/ml (18-72 microM by dimer Mr). Results of the MALS experiment show the average in-solution Mr increases from 134 kDa to 158 kDa with increasing protein load concentration, consistent with a concentration-dependent dimer-tetramer equilibrium
763029
1.2.1.31
31000
-
Lys5 from induced E. coli cells
288201
1.2.1.31
110000
-
dimeric enzyme, gel filtration and mass spectrometry
763029
1.2.1.31
154900
-
analysis of the nucleotide sequence of the 5.1-kb region
288200
1.2.1.31
155000
-
1 * 155000, SDS-PAGE
656194
1.2.1.31
155000
-
full-length Lys2 from induced E. coli cells
288201
1.2.1.31
160000
-
gel filtration
288192
1.2.1.31
171000
-
gel filtration
656194
1.2.1.31
180000
-
gel filtration
288189
1.2.1.31
184000
-
tetrameric enzyme, gel filtration and mass spectrometry
763029
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