EC Number |
General Stability |
Reference |
---|
1.1.1.22 | 2-mercaptoethanol stabilizes |
286293 |
1.1.1.22 | fairly stable with 2 mM dithiothreitol |
286301 |
1.1.1.22 | NAD+ protects against heat inactivation |
286294 |
1.1.1.22 | stable during repeated freezing and thawing cycles with 10% glycerol |
286301 |
1.1.1.22 | the half-life of UGDH catalytic activity in vitro is reduced by mutations at the dimer interface |
740715 |
1.1.1.22 | UDPglucose protects against heat inactivation |
286294 |
1.1.1.22 | UDPglucose stabilizes |
286293 |
1.1.1.22 | wild-type UGDH is relatively stable to proteolysis in the apoenzyme form but is further stabilized by addition of NADH cofactor, UDP-glucose substrate, or a combination of the two to form a ternary enzyme complex |
760577 |