Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lehmann, K.C.; Gorbalenya, A.E.; Snijder, E.J.; Posthuma, C.C.
    Arterivirus RNA-dependent RNA polymerase: vital enzymatic activity remains elusive (2016), Virology, 487, 68-74.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 recombinant expression of C-terminally His-tagged RNA-dependent RNA polymerase (RdRp) as the C-terminal domain of nonstructural protein 9 (nsp9) in Escherichia coli strain BL21 (DE3) equine arteritis virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Mg2+ required equine arteritis virus
2.7.7.48 Mn2+ essentially required for catalytic activity equine arteritis virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 equine arteritis virus
-
 an arterivirus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48 recombinant C-terminally His-tagged RdRp as the C-terminal domain of nonstructural protein 9 from Escherichia coli strain BL21 (DE3) by cobalt affinity chromatography and gel filtration equine arteritis virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 additional information in vitro, protein nsp9 of equine arteritis virus shows weak de novo polymerase activity on homopolymeric RNA templates. The RNA-synthesizing activities observed in de novo and primer-dependent polymerase and terminal transferase assays cannot be attributed to recombinant EAV nsp9-RdRp. The polymerase assay employing 32P-labeled NTPs is sensitive enough to detect the activity of trace amounts of contaminating T7 RNA polymerase. This polymerase is also able to act on templates lacking the established T7 promoter requirements equine arteritis virus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 RDRP
-
 equine arteritis virus
2.7.7.48 RNA-dependent RNA polymerase
-
 equine arteritis virus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.48 30
-
 assay at equine arteritis virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.48 8
-
 assay at equine arteritis virus

General Information

EC Number General Information Comment Organism
2.7.7.48 additional information the RNA-dependent RNA polymerase (RdRp) in equine arteritis virus is expressed as the C-terminal domain of nonstructural protein 9 (nsp9) equine arteritis virus