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Literature summary extracted from

  • Gagnon, C.; Harbour, D.; Camato, R.
    Purification and characterization of protein methylesterase from rat kidney (1984), J. Biol. Chem., 259, 10212-10215.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.61 Divalent ions
-
 Rattus norvegicus
3.1.1.61 SDS
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.61 0.0059
-
 ovalbumin methyl ester pH 6.0, 37°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.61 31000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.61 additional information Rattus norvegicus in prokaryotes it plays a crucial role in bacterial chemotaxis ?
-
 ?
3.1.1.61 additional information Rattus norvegicus in eukaryotes the enzyme is possibly involved in leukocyte chemotaxis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.61 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.61
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.61 brain
-
 Rattus norvegicus
-
3.1.1.61 kidney
-
 Rattus norvegicus
-
3.1.1.61 liver
-
 Rattus norvegicus
-
3.1.1.61 spermatozoon from caput epididymidis, level declines more than 20fold during maturation as spermatozoa acquire motility and capacity to fertilize Rattus norvegicus
-
3.1.1.61 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.61 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.1.61 -20°C, 10 mM sodium acetate buffer, pH 4.35, 50% glycerol, 0.1 mM DTT, purified enzyme stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.61 additional information in prokaryotes it plays a crucial role in bacterial chemotaxis Rattus norvegicus ?
-
 ?
3.1.1.61 additional information in eukaryotes the enzyme is possibly involved in leukocyte chemotaxis Rattus norvegicus ?
-
 ?
3.1.1.61 ovalbumin methyl ester + H2O
-
 Rattus norvegicus ovalbumin + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.61 monomer * 31000 SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.61 4
-
-
 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.61 2 10 30 min, stable Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.61 Rattus norvegicus isoelectric focusing
-
 4.45