Literature summary for 5.6.2.4 extracted from

Karow, J.K.; Chakraverty, R.K.; Hickson, I.D.
The Blooms syndrome gene product is a 3'-5' DNA helicase (1997), J. Biol. Chem., 272, 30611-30614 .

Search for more literature for 5.6.2.4

Activating Compound

Activating Compound	Comment	Organism	Structure
DNA	stimulates the ATPase activity of the enzyme	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Saccharomyces cerevisiae strain JEL1	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on, 5 mM used in assay conditions	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel chelate affinity column chromatography, and Superdex 200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
single-stranded M13 viral form DNA annealed to a radiolabeled 91-mer oligonucleotide	the enzyme possesses an ATPase activity that is strongly stimulated by either single- or double-stranded DNA. Moreover, the enzyme exhibits ATP- and Mg2+-dependent DNA helicase activity that displays 3'-5' directionality	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 180000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
BLM	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
65	-	the enzyme is inactive after 10 min at 65°C	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	enzyme gene mutations lead to Bloom's syndrome, an autosomal recessive condition characterized by short stature, immunodeficiency, and a greatly elevated frequency of many types of cancer	Homo sapiens

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Release 2023.1 (January 2023)