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Literature summary for 5.6.2.4 extracted from
- The interplay of DNA binding, ATP hydrolysis and helicase activities of the archaeal MCM helicase (2011), Biochem. J., 436, 409-414.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E422A | up to 2fold higher affinity for the DNA substrate than the wild-type enzyme. ATPase activity and helicase activity are similar to wild-type enzyme | Saccharolobus solfataricus |
| E422R | has the same affinity as the wild-type enzyme. No ability to hydrolyse ATP, no helicase activity | Saccharolobus solfataricus |
| E422R/R329E | mutant lacks detectable ATPase and helicase activities | Saccharolobus solfataricus |
| L565D | has the same affinity as the wild-type enzyme. ATPase properties similar to wild-type enzyme, significant stimulation of helicase activity | Saccharolobus solfataricus |
| L565K | up to 2fold lower affinity for the DNA substrate than the wild-type enzyme. ATPase properties similar to wild-type enzyme, significant stimulation of helicase activity | Saccharolobus solfataricus |
| R329E | up to 2fold lower affinity for the DNA substrate than the wild-type enzyme. Low and non-DNA-stimulated ATPase activity. Helicase activity is similar to wild-type enzyme | Saccharolobus solfataricus |
| R331I | has the same affinity as the wild-type enzyme. No ability to hydrolyse ATP, no helicase activity | Saccharolobus solfataricus |
| R331K | up to 2fold higher affinity for the DNA substrate than the wild-type enzyme. ATPase properties similar to wild-type enzyme. Helicase activity at approximately half the level of wild-type enzyme | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q9UXG1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Arg329 is a key residue in the communication between the DNA-binding site of SsoMCM and the trans component of the ATPase active site | Saccharolobus solfataricus | ADP + phosphate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCM helicase | - |
Saccharolobus solfataricus |
| minichromosome maintenance protein helicase | - |
Saccharolobus solfataricus |
| SSO0774 | locus name | Saccharolobus solfataricus |
| SsoMCM | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Saccharolobus solfataricus |
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Release 2023.1 (January 2023)
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