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Literature summary for 5.6.2.4 extracted from

  • Prakash, R.; Krejci, L.; van Komen, S.; Schürer, A.K.; Kramer, W.; Sung, P.
    Saccharomyces cerevisiae MPH1 gene, required for homologous recombination-mediated mutation avoidance, encodes a 3' to 5' DNA helicase (2005), J. Biol. Chem., 280, 7854-7860.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
heterotrimeric single-stranded DNA binding protein replication protein A enhances DNA helicase activity of Mph1 Saccharomyces cerevisiae
single-stranded DNA the enzyme requires single-stranded DNA for activation Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
the carboxyl-terminal His6 epitope is attached to the MPH1-coding sequence and the tagged gene is placed under the galactose inducible GAL1 promoter in the vector pYES Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
KCl 30% inhibition occurs when KCl concentration is increased from 15 to 200 mM Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximal activity is obtained with Mg2+, whereas Co2+ and Ca2+ are much less effective in this regard. No activity is observed with Mn2+ or Zn2+ Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P40562
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-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O DNA helicase activity has a 3' to 5' polarity with respect to the DNA strand on which this protein translocates Saccharomyces cerevisiae ADP + phosphate
-
?

Synonyms

Synonyms Comment Organism
3' to 5' DNA helicase
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Saccharomyces cerevisiae
MPH1
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Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6 8.4
-
Saccharomyces cerevisiae