Activating Compound | Comment | Organism | Structure |
---|---|---|---|
heterotrimeric single-stranded DNA binding protein replication protein A | enhances DNA helicase activity of Mph1 | Saccharomyces cerevisiae | |
single-stranded DNA | the enzyme requires single-stranded DNA for activation | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
the carboxyl-terminal His6 epitope is attached to the MPH1-coding sequence and the tagged gene is placed under the galactose inducible GAL1 promoter in the vector pYES | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | 30% inhibition occurs when KCl concentration is increased from 15 to 200 mM | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal activity is obtained with Mg2+, whereas Co2+ and Ca2+ are much less effective in this regard. No activity is observed with Mn2+ or Zn2+ | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P40562 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | DNA helicase activity has a 3' to 5' polarity with respect to the DNA strand on which this protein translocates | Saccharomyces cerevisiae | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3' to 5' DNA helicase | - |
Saccharomyces cerevisiae |
MPH1 | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.4 | - |
Saccharomyces cerevisiae |