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Literature summary for 5.6.2.4 extracted from
- Purification and characterization of a DNA helicase from pea chloroplast that translocates in the 3'-to-5' direction (1996), Eur. J. Biochem., 238, 54-63.
General Stability
| General Stability | Organism |
|---|---|
| trypsin destroys activity | Pisum sativum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | 45 mM | Pisum sativum |  |
| ATP | 10 mM | Pisum sativum | |
| daunorubicin | 0.01 mM, completely inhibits DNA helicase reaction | Pisum sativum | |
| dsDNA | 0.01 mM | Pisum sativum | |
| EDTA | 5 mM | Pisum sativum | |
| histone H1 | 0.001 mg/ml, inhibits of the DNA helicase activity | Pisum sativum | |
| KCl | 400 mM | Pisum sativum | |
| Nogalamycin | 0.01 mM, completely inhibits DNA helicase reaction | Pisum sativum | |
| potassium phosphate | - |
Pisum sativum | |
| RNA | 0.01 mM | Pisum sativum | |
| ssDNA | 0.01 mM | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Pisum sativum | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ or Mn2+ required, optimum concentration of MgCl2 is 1 mM | Pisum sativum | |
| Mn2+ | Mg2+ or Mn2+ required | Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
2 * 68000, SDS-PAGE | Pisum sativum |
| 136000 | - |
gel filtration | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Pisum sativum | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, inactivation after prolonged storage | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | ATP is the most active NTP. DNA helicase unwinds DNA unidirectionally from 3 to 5. DNA helicase can unwind a 17-bp duplex whether it has unpaired single-stranded tails at both the 5 end and 3 end, at the 5 end or at the 3 end only, or at neither end. However, it fails to act on a blunt-ended 17-bp duplex DNA | Pisum sativum | ADP + phosphate | - |
? | |
| dATP + H2O | dATP shows 25% of the activity compared to ATP. DNA helicase unwinds DNA unidirectionally from 3 to 5 | Pisum sativum | dADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 68000, SDS-PAGE | Pisum sativum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
1 min, inactivated | Pisum sativum |
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