Literature summary for 5.6.2.3 extracted from

Soni, R.K.; Mehra, P.; Choudhury, N.R.; Mukhopadhyay, G.; Dhar, S.K.
Functional characterization of Helicobacter pylori DnaB helicase (2003), Nucleic Acids Res., 31, 6828-6840 .

Search for more literature for 5.6.2.3

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	-	Helicobacter pylori

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in an Escherichia coli DnaB temperature-sensitive mutant	Helicobacter pylori

Inhibitors

Inhibitors	Comment	Organism	Structure
KCl	helicase activity is inhibited at concentrations of more than 25 mM KCl	Helicobacter pylori

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	1.5 mM used in assay conditions, 2 mM Mg2+ is found to be optimal for the helicase activity	Helicobacter pylori
additional information	the order of divalent cation preference on helicase activity is as follows: Mg2+> Mn2+ > Ca2+ > Co2+ > Zn2+. No activity is measured in the absence of divalent cations	Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
360000	-	gel filtration	Helicobacter pylori

Organism

Organism	UniProt	Comment	Textmining
Helicobacter pylori	-	-	-
Helicobacter pylori 26695	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography, Q Sepharose column chromatography, agarose-immobilized GST bead chromatography	Helicobacter pylori

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
partial duplex DNA	the enzyme shows strong 5 to 3 helicase and ATPase activity and can also use UTP, CTP, GTP and dATP as energy source, Ca2+ as a co-factor and untailed substrate for the helicase activity. When dCTP, dGTP, dTTP, ADP or ATPgammaS are included in the reaction mixture instead of ATP, no helicase activity is detected	Helicobacter pylori	?	-	?
partial duplex DNA	the enzyme shows strong 5 to 3 helicase and ATPase activity and can also use UTP, CTP, GTP and dATP as energy source, Ca2+ as a co-factor and untailed substrate for the helicase activity. When dCTP, dGTP, dTTP, ADP or ATPgammaS are included in the reaction mixture instead of ATP, no helicase activity is detected	Helicobacter pylori 26695	?	-	?

Subunits

Subunits	Comment	Organism
homohexamer	6 * 55000, His-tagged enzyme, SDS-PAGE	Helicobacter pylori

Synonyms

Synonyms	Comment	Organism
DnaB helicase	-	Helicobacter pylori

Cofactor

Cofactor	Comment	Organism	Structure
ATP	2 mM used in assay conditions. Helicase activity is stimulated at low ATP concentrations, reaches a peak at 2 mM ATP concentration, followed by a decrease in the activity at higher concentrations	Helicobacter pylori

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Release 2023.1 (January 2023)