Literature summary for 5.6.2.3 extracted from

Ivessa, A.S.; Zhou, J.Q.; Schulz, V.P.; Monson, E.K.; Zakian, V.A.
Saccharomyces Rrm3p, a 5' to 3' DNA helicase that promotes replication fork progression through telomeric and subtelomeric DNA (2002), Genes Dev., 16, 1383-1396 .

Search for more literature for 5.6.2.3

Protein Variants

Protein Variants	Comment	Organism
K260A	helicase-dead mutant	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, GST column chromatography, and Q Sepharose column chromatography	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
duplex DNA	partially duplex DNA substrate in which a 36-mer oligonucleotide and a 25-mer oligonucleotide are annealed at, respectively, the 5'and 3' ends of linear, single-stranded M13 DNA	Saccharomyces cerevisiae	?	-	?
additional information	enzyme Rrm3p is an ATPase and 5' to 3' DNA helicase	Saccharomyces cerevisiae	?	-	-

Synonyms

Synonyms	Comment	Organism
5' to 3' DNA helicase	-	Saccharomyces cerevisiae
Rrm3p	-	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	dependent on	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
malfunction	enzyme loss results in replication fork pausing at specific sites in subtelomeric DNA, such as at inactive replication origins, and at internal tracts of C1-3A/TG1-3 DNA	Saccharomyces cerevisiae
physiological function	the enzyme promotes replication fork progression through telomeric and subtelomeric DNA	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)