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Literature summary for 5.6.2.3 extracted from
- Plasmodium falciparum RuvB1 is an active DNA helicase and translocates in the 5'-3' direction (2013), Gene, 515, 99-109 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) Codon Plus cells | Plasmodium falciparum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Plasmodium falciparum | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 1 mM used in assay conditions | Plasmodium falciparum |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is an ATPase both in the presence and absence of DNA, and this activity increases significantly in the presence of single-stranded DNA. The enzyme also contains DNA helicase activity and translocates preferentially in 5' to 3' direction | Plasmodium falciparum | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * about 59000, calculated from amino acid sequence | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RuvB1 | - |
Plasmodium falciparum |
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Release 2023.1 (January 2023)
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