Any feedback?
Literature summary for 5.6.2.3 extracted from
- Isolation and characterisation of dhel II, a DNA helicase from Drosophila melanogaster embryos stimulated by Escherichia coli-type single-stranded-DNA-binding proteins (1996), Eur. J. Biochem., 235, 152-158 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| mitochondria1 single-stranded DNA-binding protein | the addition of Escherichia coli-like single-stranded DNA-binding proteins, in particular the mitochondria1 single-stranded DNA-binding protein, stimulates the unwinding activity of the helicase at least 10fold on substrates greater than 200 nucleotides | Drosophila melanogaster |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 1 mM used in assay conditions | Drosophila melanogaster |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
gel filtration | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| MonoQ column chromatography and MonoS column chromatography | Drosophila melanogaster |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Drosophila melanogaster | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 61-nucleotide DNA | - |
Drosophila melanogaster | ? | - |
? | |
| additional information | the helicase unwinds DNA 5' to 3'and has a preference for purine triphosphates as cofactors for the unwinding reaction (GTP/dGTP are better substrates than ATP/dATP. CTP/dCTP support the reaction at higher concentrations, while UTP and dTTP can hardly be utilised at all) | Drosophila melanogaster | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 120000, SDS-PAGE | Drosophila melanogaster |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dhel 11 | - |
Drosophila melanogaster |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
