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Literature summary for 5.6.2.3 extracted from

  • Saikrishnan, K.; Powell, B.; Cook, N.; Webb, M.; Wigley, D.
    Mechanistic basis of 5'-3' translocation in SF1B helicases (2009), Cell, 137, 849-859 .
    View publication on PubMed
Search for more literature for 5.6.2.3

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with single-stranded DNA in the presence and absence of the ATP analog ADPNP, hanging drop vapor diffusion method, using 10%-18% (w/v) PEG 8K, 2%-6% (v/v) ethylene glycol, 100 mM Tris (pH 8.5) Deinococcus radiodurans

Protein Variants

Protein Variants Comment Organism
N596A the mutant is defective in helicase activity (greater than four orders of magnitude compared to the wild type enzyme) Deinococcus radiodurans
N604A the mutant is defective in helicase activity (greater than four orders of magnitude compared to the wild type enzyme) Deinococcus radiodurans
Y598A the mutant is defective in helicase activity (10fold compared to the wild type enzyme) Deinococcus radiodurans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Deinococcus radiodurans

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans Q9RT63
-
-
Deinococcus radiodurans ATCC 13939 Q9RT63
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows 5`-3` translocation directionality Deinococcus radiodurans ?
-
-
additional information the enzyme shows 5`-3` translocation directionality Deinococcus radiodurans ATCC 13939 ?
-
-

Synonyms

Synonyms Comment Organism
RecD2
-
 Deinococcus radiodurans

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Deinococcus radiodurans