Literature summary for 5.6.2.3 extracted from

Huang, Q.; Liu, L.; Liu, J.; Ni, J.; She, Q.; Shen, Y.
Efficient 5'-3' DNA end resection by HerA and NurA is essential for cell viability in the crenarchaeon Sulfolobus islandicus (2015), BMC Mol. Biol., 16, 2-2 .

Search for more literature for 5.6.2.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3)-Codon-Plus-RIL cells	Sulfolobus islandicus

Protein Variants

Protein Variants	Comment	Organism
D176E	mutant with reduced ATPase activity compared to the wild type enzyme	Sulfolobus islandicus
D176N	mutant with severely reduced ATPase activity compared to the wild type enzyme	Sulfolobus islandicus
E356D	mutant with reduced ATPase activity compared to the wild type enzyme	Sulfolobus islandicus
E356Q	mutant with severely reduced ATPase activity compared to the wild type enzyme	Sulfolobus islandicus
K154R	the mutant has no ATPase activity	Sulfolobus islandicus
R381K	the mutant has no ATPase activity	Sulfolobus islandicus

Organism

Organism	UniProt	Comment	Textmining
Sulfolobus islandicus	-	-	-
Sulfolobus islandicus Rey15A	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration	Sulfolobus islandicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	helicase HerA also has ATPase activity	Sulfolobus islandicus	?	-	-
additional information	helicase HerA also has ATPase activity	Sulfolobus islandicus Rey15A	?	-	-

Synonyms

Synonyms	Comment	Organism
HerA	-	Sulfolobus islandicus

General Information

General Information	Comment	Organism
physiological function	efficient 5'-3' DNA end resection by the helicase HerA/nuclease NurA complex is essential for cell viability in Sulfolobus islandicus	Sulfolobus islandicus

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Release 2023.1 (January 2023)