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Literature summary for 5.6.2.3 extracted from

  • Liu, H.; Yan, P.; Fanning, E.
    Human DNA helicase B functions in cellular homologous recombination and stimulates Rad51-mediated 5-3 heteroduplex extension in vitro (2015), PLoS ONE, 10, e0116852 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.3

Cloned(Commentary)

Cloned (Comment) Organism
ectopically expressed HDHB colocalizes with Rad51, Rad52, RPA, and ssDNA Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information ectopically expressed HDHB colocalizes with Rad51, Rad52, RPA, and ssDNA Homo sapiens
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Homo sapiens
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 ADP + phosphate
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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Homo sapiens Q8NG08
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Source Tissue

Source Tissue Comment Organism Textmining
colonic cancer cell
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 Homo sapiens
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HCT-116 cell
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 Homo sapiens
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osteosarcoma cell
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 Homo sapiens
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U2-OS cell
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 Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
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 Homo sapiens ADP + phosphate
-
 ?
additional information in vitro, the enzyme stimulates Rad51-mediated heteroduplex extension in 5'-3' direction Homo sapiens ?
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-

Synonyms

Synonyms Comment Organism
5'-3' DNA helicase
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 Homo sapiens
DNA helicase B
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 Homo sapiens
HDHB
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 Homo sapiens
HELB
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 Homo sapiens

General Information

General Information Comment Organism
evolution DNA helicase B is a robust 5'-3' superfamily I DNA helicase conserved among vertebrates Homo sapiens
malfunction HDHB silencing results in reduced sister chromatid exchange, impaired homologous recombination repair, and delayed RPA late stage foci formation induced by ionizing radiation. A helicase-defective mutant HDHB fails to promote this reaction. HDHB-depleted cells have fewer sister chromatid exchange events and impaired homologous recombination repair induced by I-SceI cleavage on a chromosomal recombination reporter cassette. Ectopically expressed HDHB colocalizes with Rad51, Rad52, RPA and ssDNA. The ionizing radiation (IR)-induced RPA late-stage foci formation is reduced in HDHB-depleted cells, while Rad51 and gammaH2AX foci formation is not affected by HDHB silencing Homo sapiens
malfunction enzyme silencing results in reduced sister chromatid exchange, impaired homologous recombination repair, and delayed replication protein A late-stage foci formation induced by ionizing radiation Homo sapiens
physiological function human DNA helicase B (HDHB) is a robust 5'-3' DNA helicase which accumulates on chromatin in cells exposed to DNA damage. HDHB facilitates cellular recovery from replication stress, it plays a role in DNA damage response. In vitro, HDHB stimulates Rad51-mediated heteroduplex extension in 5'-3' direction. HDHB promotes homologous recombination in vivo and stimulates 5'-3' heteroduplex extension during Rad51-mediated strand exchange in vitro Homo sapiens
physiological function the enzyme facilitates cellular recovery from replication stress. The enzyme promotes homologous recombination in vivo and stimulates 5'-3' heteroduplex extension during Rad51-mediated strand exchange in vitro Homo sapiens