Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.2.3 extracted from

  • Sabouri, N.; Capra, J.; Zakian, V.
    The essential Schizosaccharomyces pombe Pfh1 DNA helicase promotes fork movement past G-quadruplex motifs to prevent DNA damage (2015), BMC Biol., 12, 101 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.3

Localization

Localization Comment Organism GeneOntology No. Textmining
chromatin
-
 Schizosaccharomyces pombe 785
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Schizosaccharomyces pombe
-
 ADP + phosphate
-
 ?
ATP + H2O Schizosaccharomyces pombe ATCC 24843
-
 ADP + phosphate
-
 ?
ATP + H2O Schizosaccharomyces pombe 972
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe Q9UUA2
-
-
Schizosaccharomyces pombe 972 Q9UUA2
-
-
Schizosaccharomyces pombe ATCC 24843 Q9UUA2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Schizosaccharomyces pombe ADP + phosphate
-
 ?
ATP + H2O
-
 Schizosaccharomyces pombe ATCC 24843 ADP + phosphate
-
 ?
ATP + H2O
-
 Schizosaccharomyces pombe 972 ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Pfh1
-
 Schizosaccharomyces pombe
Pfh1 DNA helicase
-
 Schizosaccharomyces pombe
Pif1 DNA helicase
-
 Schizosaccharomyces pombe
Pif1 family helicase
-
 Schizosaccharomyces pombe
PIF1 helicase
-
 Schizosaccharomyces pombe

General Information

General Information Comment Organism
evolution Pif1 family helicases are found in the genomes of organisms from all three kingdoms. Most eukaryotes, including Schizosaccharomyces pombe and humans, encode a single Pif1 family helicase, while Saccharomyces cerevisiae encodes two, ScPif1 and ScRrm3 Schizosaccharomyces pombe
malfunction in the absence of functional Pfh1, unresolved G4 structures cause fork pausing and DNA damage. In the absence of Pfh1, G4 motifs associated with replication fork stalling are more likely to result in double strand breaks. A subset of G4 motifs have high Cdc20 binding indicative of replication fork pausing in Pfh1-depleted cells Schizosaccharomyces pombe
additional information G-quadruplexes (G4s) are stable non-canonical DNA secondary structures consisting of stacked arrays of four guanines, each held together by Hoogsteen hydrogen bonds. Sequences with the ability to form these structures in vitro, G4 motifs, are found throughout bacterial and eukaryotic genomes. Genomic distribution and evolutionary conservation of G4 motifs in four fission yeast species are determined via genome-wide search for DNA sequences with the potential to form G4 structures, overview. Identification of all sequences that contain four runs of three or more guanine base pairs (bp), G-islands, separated by loop regions of no more than 25 bp (G > or = 3 N1 25)3 G > or = 3. Regions with more than four G-islands separated by < or = 25 bp are counted as a single G4 motif. Excluding repetitive DNA, the Schizosaccharomyces pombe genome contains 446 G4 motifs that match this query pattern with a density of 0.036 G4 motifs/kilobase Schizosaccharomyces pombe
physiological function Pfh1, the Schizosaccharomyces pombe Pif1 helicase, is essential for maintenance of both the nuclear and mitochondrial genomes. Many G4 motifs in the Schizosaccharomyces pombe genome are associated with Pfh1. Pfh1-associated G4 motifs are located on the transcribed strand of highly transcribed genes significantly more often than expected, suggesting that Pfh1 has a function in replication or transcription at these sites. DNA damage occurs near G4 motifs. Pif1 family helicases unwind G4 structures robustly in vitro and suppress G4-induced DNA damage Schizosaccharomyces pombe