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Literature summary for 5.6.2.3 extracted from

  • Ahmad, M.; Tuteja, R.
    Plasmodium falciparum RuvB1 is an active DNA helicase and translocates in the 5'-3' direction (2013), Gene, 515, 99-109.
    View publication on PubMed
Search for more literature for 5.6.2.3

Activating Compound

Activating Compound Comment Organism Structure
ssDNA activates the ATPase activity of the enzyme Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant His-tagged enzyme PfRuvB1 in Escherichia coli strain BL21(DE3) and BL21 codon plus in inclusion bodies, a heat shock protocol helps to obtain the PfRuvB1 expression in the soluble fraction Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme PfRuvB1 localizes mainly to the nucleus Plasmodium falciparum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 x * 59000, about, sequence calculation Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Plasmodium falciparum
-
 ADP + phosphate
-
 ?
additional information Plasmodium falciparum enzyme RuvB1 is an active DNA helicase and translocates in the 5'-3' direction ?
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum J7HDV0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme PfRuvB1 from Escherichia coli strain BL21(DE3) and BL21 codon plus soluble fraction by nickel affinity chromatography to homogeneity Plasmodium falciparum

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme PfRuvB1 is constitutively expressed during all the stages of intraerythrocytic cycle of Plasmodium. falciparum Plasmodium falciparum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Plasmodium falciparum ADP + phosphate
-
 ?
ATP + H2O PfRuvB1 is an ATPase and this activity increases significantly in the presence of ssDNA, PfRuvB1 also contains DNA helicase activity and translocates preferentially in 5' to 3' direction, substrates are M13 mp19 ssDNA and oligonucleotides Plasmodium falciparum ADP + phosphate
-
 ?
additional information enzyme RuvB1 is an active DNA helicase and translocates in the 5'-3' direction Plasmodium falciparum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 59000, about, sequence calculation Plasmodium falciparum

Synonyms

Synonyms Comment Organism
RuvB1
-
 Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Plasmodium falciparum

General Information

General Information Comment Organism
evolution the RuvB family of protein contains two similar kinds of proteins i.e. RuvB1 and RuvB2 from yeast to human. Plasmodium falciparum contains three RuvB proteins (PfRuvB1, PfRuvB2 and PfRuvB3) instead of two. These proteins belong to the AAA+ class of proteins and are critical components of several multiprotein complexes involved in diverse cellular activities. PfRuvB1 shows considerable homology with human as well as yeast RuvB1 and contains Walker motif A and Walker motif B. Plasmodium falciparum