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Literature summary for 5.6.2.3 extracted from
- The Arabidopsis At1g30680 gene encodes a homologue to the phage T7 gp4 protein that has both DNA primase and DNA helicase activities (2013), BMC Plant Biol., 13, 36.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene At1g30680, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | the Arabidopsis Twinkle homologue enzyme is dual targeted to mitochondria and chloroplasts | Arabidopsis thaliana | 9507 | - |
| mitochondrion | the Arabidopsis Twinkle homologue enzyme is dual targeted to mitochondria and chloroplasts | Arabidopsis thaliana | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Arabidopsis thaliana |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
x * 80000, about, sequence calculation, x * 74000, recombinant enzyme, SDS-PAGE | Arabidopsis thaliana |
| 80000 | - |
x * 80000, about, sequence calculation, x * 74000, recombinant enzyme, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Arabidopsis thaliana | - |
ADP + phosphate | - |
? | |
| additional information | Arabidopsis thaliana | the enzyme has both DNA primase and DNA helicase activities. The helicase utilizes ATP hydrolysis to separate the DNA double helix into individual strands. DNA primase catalyzes the formation of short RNA oligonucleotides used as primers to begin DNA synthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | B5X582 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | young | Arabidopsis thaliana | - |
| shoot | apex tissue | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Arabidopsis thaliana | ADP + phosphate | - |
? | |
| additional information | the enzyme has both DNA primase and DNA helicase activities. The helicase utilizes ATP hydrolysis to separate the DNA double helix into individual strands. DNA primase catalyzes the formation of short RNA oligonucleotides used as primers to begin DNA synthesis | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, about, sequence calculation, x * 74000, recombinant enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Twinkle homologue | - |
Arabidopsis thaliana |
| Twinkle protein | - |
Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | phylogenetic analysis of amino acid sequences of Twinkle homologues from several plants and other species shows that the Arabidopsis and plant homologues are closely clustered and are most similar to the bacteriophage T7 gp4 protein | Arabidopsis thaliana |
| physiological function | the enzyme plays a role in organelle DNA replication. The helicase utilizes ATP hydrolysis to separate the DNA double helix into individual strands. DNA primase catalyzes the formation of short RNA oligonucleotides used as primers to begin DNA synthesisesis | Arabidopsis thaliana |
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