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Literature summary for 5.6.2.3 extracted from

  • Jemt, E.; Farge, G.; Baeckstroem, S.; Holmlund, T.; Gustafsson, C.M.; Falkenberg, M.
    The mitochondrial DNA helicase TWINKLE can assemble on a closed circular template and support initiation of DNA synthesis (2011), Nucleic Acids Res., 39, 9238-9249.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in SF9 cells as His-tagged fusion proteins lacking the N-terminal mitochondrial targeting signal using the baculovirus expression system Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the loading of TWINKLE onto a circular ssDNA in vitro is examined. TWINKLE is able to load onto circular ssDNA without the help of a loading factor and can support initiation of DNA replication on a closed circular dsDNA substrate in combination with only mitochondrial DNA polymerase gamma ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the loading of TWINKLE onto a circular ssDNA in vitro is examined. TWINKLE is able to load onto circular ssDNA without the help of a loading factor and can support initiation of DNA replication on a closed circular dsDNA substrate in combination with only mitochondrial DNA polymerase gamma Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
TWINKLE DNA helicase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens