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Literature summary for 5.6.2.3 extracted from
- Human DNA helicase B (HDHB) binds to replication protein A and facilitates cellular recovery from replication stress (2012), J. Biol. Chem., 287, 6469-6481.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Hi5 insect cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | His-tagged HDHB fragments incubated with glutathione beads bind to GST-RPA70N. HDHB residues 394-958 and 459-811 bind specifically to GST-RPA70N beads, demonstrating a specific interaction between HDHB 459-811 and RPA70N | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | HDHB interacts with the N-terminal domain of replication protein A 70-kDa subunit | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
| additional information | HDHB interacts with the N-terminal domain of replication protein A 70-kDa subunit | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HDHB | - |
Homo sapiens |
| human DNA helicase B | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | HDHB knockdown impairs recovery from replication stress | Homo sapiens |
| physiological function | cellular exposure to UV irradiation, camptothecin, or hydroxyurea induces accumulation of HDHB on chromatin in a dose- and time-dependent manner, preferentially in S phase cells. Replication stress-induces recruitment of HDHB to chromatin is independent of checkpoint signaling but correlates with the level of replication protein A recruited to chromatin | Homo sapiens |
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Release 2023.1 (January 2023)
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