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Literature summary for 5.6.2.3 extracted from

  • Wu, Y.; Sommers, J.A.; Loiland, J.A.; Kitao, H.; Kuper, J.; Kisker, C.; Brosh, R.M.
    The Q Motif of Fanconi Anemia Group J Protein (FANCJ) DNA Helicase Regulates Its Dimerization, DNA Binding, and DNA Repair Function (2012), J. Biol. Chem., 287, 21699-21716.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in insect cells using the baculovirus expression system Homo sapiens

Protein Variants

Protein Variants Comment Organism
A349P expression of a FANCJ mutant protein with a pathogenic A349P amino acid substitution in a wild-type background exerts a dominant negative effect on resistance to DNA cross-linking agents or TMS Homo sapiens
DELTAQ25 mutant bearing a deletion of Q25 shows a similar phenotype as Q25A Homo sapiens
K52R mutant is seriously compromised in its ATPase activity, kcat (ATP) highly decreased compared to wild-type Homo sapiens
Q25A Q25A mutation of the invariant glutamine in the Q motif abolishes its ability to complement cisplatin or telomestatin sensitivity of a fancj null cell line and exerts a dominant negative effect. Mutant shows impaired FANCJ helicase activity and ATPase activity but displays ATP binding and temperature-induced unfolding transition similar to wild-type. Mutant exists only as a monomer. Km (ATP) increased compared to wild-type, kcat 10fold decreased compared to wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.89
-
 ATP wild-type, pH 7.4, 30°C, in the presence of M13 ssDNA Homo sapiens
1.4
-
 ATP mutant DELTA Q25, pH 7.4, 30°C, in the presence of M13 ssDNA Homo sapiens
1.5
-
 ATP mutant Q25A, pH 7.4, 30°C, in the presence of M13 ssDNA Homo sapiens
1.6
-
 ATP wild-type, pH 7.4, 30°C, in the absence of DNA Homo sapiens
1.8
-
 ATP mutant Q25A, pH 7.4, 30°C, in the absence of DNA Homo sapiens
1.9
-
 ATP mutant DELTA Q25, pH 7.4, 30°C, in the absence of DNA Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BX63
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Homo sapiens ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer FANCJ exists as molecular weight species corresponding to a monomer and a dimer, and the dimeric form displays a higher specific activity for ATPase and helicase, as well as greater DNA binding Homo sapiens
monomer FANCJ exists as molecular weight species corresponding to a monomer and a dimer, and the dimeric form displays a higher specific activity for ATPase and helicase, as well as greater DNA binding Homo sapiens

Synonyms

Synonyms Comment Organism
FANCJ
-
 Homo sapiens
Fanconi Anemia Group J Protein
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 ATP mutant DELTA Q25, pH 7.4, 30°C, in the absence of DNA Homo sapiens
0.075
-
 ATP mutant K52R, pH 7.4, 30°C, in the presence of M13 ssDNA Homo sapiens
0.085
-
 ATP mutant K52R, pH 7.4, 30°C, in the presence of M13 ssDNA Homo sapiens
0.155
-
 ATP mutant Q25A, pH 7.4, 30°C, in the absence of DNA Homo sapiens
0.2
-
 ATP wild-type, pH 7.4, 30°C, in the absence of DNA Homo sapiens
1.68
-
 ATP mutant DELTA Q25, pH 7.4, 30°C, in the presence of M13 ssDNA Homo sapiens
3.6
-
 ATP mutant Q25A, pH 7.4, 30°C, in the presence of M13 ssDNA Homo sapiens
43.3
-
 ATP wild-type, pH 7.4, 30°C, in the presence of M13 ssDNA Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens