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Literature summary for 5.6.2.3 extracted from
- One-step column purification of herpes simplex virus 1 helicase-primase subcomplex using C-terminally his-tagged UL5 subunit (2009), Virus Genes, 39, 19-29.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His6-tagged DNA helicase expressed via recombinant baculovirus | Human alphaherpesvirus 1 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Human alphaherpesvirus 1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| one-step column purification of helicaseprimase subcomplex (helicase-primase enzyme complex consisting of UL5 and UL52 gene functions) using C-terminally His-tagged UL5 subunit | Human alphaherpesvirus 1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | 5'-3' unwinding activity, enzymatic functions of the two subunit helicase-primase complex (enzyme complex consisting of UL5 and UL52 gene functions): DNA-dependent ATPase, DNA primase, and DNA helicase activities | Human alphaherpesvirus 1 | ADP + phosphate | - |
? |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Human alphaherpesvirus 1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Human alphaherpesvirus 1 |
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