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Literature summary for 5.6.2.3 extracted from

  • Biswas, E.E; Nagele, R.G.; Biswas, S.
    A novel human hexameric DNA helicase: expression, purification and characterization. (2001), Nucleic Acids Res., 29, 1733-1740.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.3

Activating Compound

Activating Compound Comment Organism Structure
additional information no significant stimulation by Escherichia coli ssDNA-binding protein Homo sapiens
replication protein A from yeast or human Homo sapiens
single-stranded DNA more than 5fold stimulation of ATPase activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 ATP
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
 6 * 116000, SDS-PAGE Homo sapiens
600000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P38935
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell maximal level of expression is observed in late G1/early S phase Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O DNA unwinding in 5' to 3' direction Homo sapiens ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 116000, SDS-PAGE Homo sapiens