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Literature summary for 5.6.2.3 extracted from
- Schizosaccharomyces pombe pfh1+ encodes an essential 5' to 3' DNA helicase that is a member of the PIF1 subfamily of DNA helicases (2002), Mol. Biol. Cell, 13, 2180-2191.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K337A | K337A and the K337R alleles are unable to supply the essential function of Pfh1p | Schizosaccharomyces pombe |
| K337R | K337A and the K337R alleles are unable to supply the essential function of Pfh1p | Schizosaccharomyces pombe |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Schizosaccharomyces pombe | 5' to 3' DNA helicase. ATPase/helicase activity of Pfh1p is essential. Maintenance of telomeric DNA is not the sole essential function of Pfh1p. Although mutant spores depleted for Pfh1p proceed through S phase, they arrest with a terminal cellular phenotype consistent with a postinitiation defect in DNA replication. Telomeric DNA is modestly shortened in the absence of Pfh1p | ADP + phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | Q9UUA2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | 5' to 3' DNA helicase. ATPase/helicase activity of Pfh1p is essential. Maintenance of telomeric DNA is not the sole essential function of Pfh1p. Although mutant spores depleted for Pfh1p proceed through S phase, they arrest with a terminal cellular phenotype consistent with a postinitiation defect in DNA replication. Telomeric DNA is modestly shortened in the absence of Pfh1p | Schizosaccharomyces pombe | ADP + phosphate | - |
? | |
| ATP + H2O | 5' to 3' DNA helicase | Schizosaccharomyces pombe | ADP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5' to 3' DNA helicase | - |
Schizosaccharomyces pombe |
| DNA helicase | - |
Schizosaccharomyces pombe |
| Pfh1p | - |
Schizosaccharomyces pombe |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Schizosaccharomyces pombe |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Schizosaccharomyces pombe |
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Release 2023.1 (January 2023)
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