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Literature summary for 5.6.2.3 extracted from

  • Shimizu, K.; Sugino, A.
    Purification and characterization of DNA helicase III from the yeast Saccharomyces cerevisiae (1993), J. Biol. Chem., 268, 9578-9584.
    View publication on PubMed
Search for more literature for 5.6.2.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 ATP pH 7.8, 30°C Saccharomyces cerevisiae
0.25
-
 dATP pH 7.8, 30°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requires divent cation, Mg2+ or Mn2+ Saccharomyces cerevisiae
Mn2+ requires divent cation, Mg2+ or Mn2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 x * 12000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O ATP hydrolysis is required for unwinding of DNA catalyzed by the DNA helicase, the enzyme moves in the 5’ to 3‘ direction on a single-stranded DNA to catalyze unwinding of double-stranded regions of DNA in the 3' to 5' direction Saccharomyces cerevisiae ADP + phosphate
-
 ?
dATP + H2O ATP hydrolysis is required for unwinding of DNA catalyzed by the DNA helicase, the enzyme moves in the 5’ to 3‘ direction on a single-stranded DNA to catalyze unwinding of double-stranded regions of DNA in the 3' to 5' direction. dATP shows 95% of the activity with ATP Saccharomyces cerevisiae dADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 12000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
DNA helicase III
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Saccharomyces cerevisiae