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Literature summary for 5.6.2.3 extracted from

  • Biswas, E.E.; Barnes, M.H.; Moir, D.T.; Biswas, S.B.
    An essential DnaB helicase of Bacillus anthracis: identification, characterization, and mechanism of action (2009), J. Bacteriol., 191, 249-260.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.3

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus anthracis

Inhibitors

Inhibitors Comment Organism Structure
M13mp19 ssDNA ATPase activity is slightly stimulated by ssDNA, and only M13mp19 ssDNA stimulates it significantly (increase in Vmax) Bacillus anthracis
ssDNA ATPase activity is slightly stimulated by ssDNA, and only M13mp19 ssDNA stimulats it significantly Bacillus anthracis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.152
-
 ATP pH 7.5, 37°C, addition of 60-mer oligonucleotide Bacillus anthracis
0.22
-
 ATP pH 7.5, 37°C, addition of M13mp19 ssDNA Bacillus anthracis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Bacillus anthracis

Organism

Organism UniProt Comment Textmining
Bacillus anthracis Q81JI8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O strong 5' to 3' DNA helicase activity. At both 0.1 and 0.5 mM, dATP produces comparable or slightly higher levels of unwinding than ATP Bacillus anthracis ADP + phosphate
-
 ?
dATP + H2O strong 5' to 3' DNA helicase activity. At both 0.1 and 0.5 mM, dATP produces comparable or slightly higher levels of unwinding than ATP Bacillus anthracis dADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Bacillus anthracis

Synonyms

Synonyms Comment Organism
dnaB
-
 Bacillus anthracis
DnaB helicase
-
 Bacillus anthracis